Singh, Renu and Verma, Rachna and Sumana, G. and Srivastava, Avanish Kumar and Sood, Seema and Gupta, Rajinder K. and Malhotra, B. D. (2012) Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection. Bioelectrochemistry, 86. 30-37. ISSN 1567-5394

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Abstract

The nanocomposite based on polyaniline (PANI)-iron oxide nanoparticles (nFe(3)O(4)) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe(3)O(4) (similar to 20 nm) and CNT (20-80 nm in diameter). The results of transmission electron microscopic studies show evidence of coating of PANI and nFe(3)O(4) onto the CNT. The PANI-nFe(3)O(4)-CNT/ITO nanoelectrode has been characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy studies. The biotinylated nucleic acid probe sequence consisting of 20 bases has been immobilized onto PANI-nFe(3)O(4)-CNT/ITO nanoelectrode using biotin-avidin coupling. It is shown that the PANI-nFe(3)O(4)-CNT platform based biosensor can be used to specifically detect bacteria (N. gonorrhoeae) at minute concentration as low as (1 x 10(-19) M) indicating high sensitivity within 45 s of hybridization time at 298 K by differential pulse voltammetry using methylene blue as electroactive indicator. This bacterial sensor has also been tested with 4 positive and 4 negative PCR amplicons of gonorrhoea affected patient samples. The results of these studies have implications towards the fabrication of a handheld device for Neisseria gonorrhoeae detection that may perhaps result in a decrease in the human immunodeficiency virus infections.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Elsevier
Subjects:	Biochemistry & Molecular Biology Biology Biophysics Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 11:07
Last Modified:	19 Aug 2026 11:07
URI:	https://npl.csircentral.net/id/eprint/3484

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