Singh, Renu and Matharu, Zimple and Srivastava, Avanish Kumar and Sood, Seema and Gupta, Rajinder Kumar and Malhotra, Bansi Dhar (2012) Nanostructured platform for the detection of Neisseria gonorrhoeae using electrochemical impedance spectroscopy and differential pulse voltammetry. Microchimica Acta, 177 ( 1-2). pp. 201-210. ISSN 0026-3672

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Abstract

We report on a nanocomposite based genosensor for the detection of Neisseria gonorrhoeae, a bacterium causing the sexually transmitted disease gonorrhoea. Amino-labeled probe DNA was covalently immobilized on electrochemically prepared polyaniline and iron oxide (PANI-Fe3O4) nanocomposite film on an indium tin oxide (ITO) electrode. Scanning electron microscopy, transmission electron microscopy, electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) techniques have been employed to characterize surface of the modified electrode. The genosensor has detection limits of 1x10(-15) M and 1x10(-17) M, respectively, using the EIS and DPV techniques. This biosensor can discriminate a complementary sequence from a single-base mismatch and from non-complementary DNA, and has been utilized for detection of DNA extracted from N. gonorrhoeae culture, and from patient samples with N. gonorrhoeae. It is found to exhibit good specificity for N. gonorrhoeae species and shows no response towards non-gonorrhoeae type of Neisseria species (NgNs) and other gram-negative bacterias (GNBs). The affinity constant for hybridization calculated using the Langmuir adsorption isotherm model is found to be 3.39x10(8) M-1.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Springer Verlag (Germany)
Subjects:	Chemistry > Analytical Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 11:06
Last Modified:	19 Aug 2026 11:06
URI:	https://npl.csircentral.net/id/eprint/3474

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