Singh, Chandan and Srivastava, Saurabh and Ali, Md. Azahar and Gupta, Tejendra K. and Sumana, Gajjala and Srivastava, Anchal and Mathur, R. B. and Malhotra, Bansi D. (2013) Carboxylated multiwalled carbon nanotubes based biosensor for aflatoxin detection. Sensors and Actuators B: Chemical, 185. 258-264. ISSN 0925-4005

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Abstract

We report results of studies relating to the development of an electrochemical immunosensor based on carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass. This c-MWCNTs/ITO electrode surface has been functionalized with monoclonal aflatoxin B-1 antibodies (anti-AFB(1)) for the detection of aflatoxin-B-1 using electrochemical technique. Electron microscopy, X-ray diffraction and Raman studies suggest successful synthesis of c-MWCNTs and the Fourier transform infra-red spectroscopic (FT-IR) studies reveal its carboxylic functionalized nature. The proposed immunosensor shows high sensitivity (95.2 mu A ng(-1) mL cm(-2)), improved detection limit (0.08 ng mL(-1)) in the linear detection range of 0.25-1.375 ng mL(-1). The low value of association constant (0.0915 ng mL(-1)) indicates high affinity of immunoelectrode towards aflatoxin (AFB(1)). (

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Elsevier
Subjects:	Chemistry > Analytical Chemistry Electrochemistry Instruments/ Instrumentation
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 10:40
Last Modified:	19 Aug 2026 10:40
URI:	https://npl.csircentral.net/id/eprint/3324

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