Ali, Md. Azahar and Srivastava, Saurabh and Solanki, Pratima R. and Agrawal, Ved Varun and John, Renu and Malhotra, Bansi D. (2012) Nanostructured anatase-titanium dioxide based platform for application to microfluidics cholesterol biosensor. Applied Physics Letters, 101 (8). 084105-084110. ISSN 0003-6951

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Abstract

We report results of studies relating to the fabrication of a microfluidics cholesterol sensor based on nanocrystalline anatase-titanium dioxide (ant-TiO2) film deposited onto indium tin oxide (ITO) glass. The results of response studies (optimized under the flow rate of 30 mu l/min) conducted on cholesterol oxidase (ChOx) immobilized onto crystalline ant-TiO2 nanoparticles (similar to 27 nm)/ITO microfluidics electrode reveal linearity as 1.3 to 10.3 mM and improved sensitivity of 94.65 mu A/mM/cm(2). The observed low value of K-m (0.14 mM) indicates high affinity of ChOx to cholesterol. No significant changes in current response of this microfluidics sensor are measured in the presence of different interferents.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s American Institute of Physics
Subjects:	Applied Physics/Condensed Matter
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 11:06
Last Modified:	19 Aug 2026 11:06
URI:	https://npl.csircentral.net/id/eprint/3476

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