Kalita, Prasanta and Singh, Jay and Singh, Manish Kumar and Solanki, Pratima R. and Sumana, G. and Malhotra, B. D. (2012) Ring like self assembled Ni nanoparticles based biosensor for food toxin detection. Applied Physics Letters, 100 (9). 093702-0937006. ISSN 0003-6951

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Abstract

The self-assembled ring like nickel (RnNi similar to 10-20 nm) nanoparticles have been prepared by pulsed laser ablation method and confirmed by transmission electron microscopy. These RnNi nanoparticles electrophoretically deposited onto the indium-tin-oxide (ITO) glass substrate have been functionalized with dimethyl sulfoxide (DMSO) for covalent immobilization of anti-aflatoxin (a-AfB1) monoclonal antibodies and bovine serum albumin as blocking agent. The electrochemical response studies of a-AfB1/DMSO/RnNi-film/ITO bioelectrode reveal linearity as 5-100 ng dL(-1), detection limit of 32.7 ngdL(-1), sensitivity of 0.59 mu A/ng dL(-1), and shelf-life of 60 days. The low value (1.3 x 10(14) molL(-1)) of association constant (K-a) shows high affinity towards aflatoxin.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s American Institute of Physics
Subjects:	Applied Physics/Condensed Matter
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 11:03
Last Modified:	19 Aug 2026 11:03
URI:	https://npl.csircentral.net/id/eprint/3442

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