Patel, Manoj K. and Solanki, Pratima R. and Khandelwal, Sachin and Agrawal, Ved V. and Ansari, S. G. and Malhotra, B. D. (2012) Self-assembled monolayer based electrochemical nucleic acid sensor for Vibrio cholerae detection. Journal of Physics: Conference Series, 358. 012009-012018. ISSN 1742-6588

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Abstract

Nucleic acid sensor has been fabricated by immobilization of thiolated (5' end) single stranded deoxyribonucleic acid probe (ssDNA-SH) onto gold (Au) coated glass electrode for Vibriocholeraedetection. This ssDNA-SH/Au bioelectrode characterized using atomic force microscopy (AFM), Fourier transforms infrared spectroscopy (FT-IR) and electrochemical technique, has been used for hybridization detection of genomic DNA (dsDNA/Au). This ssDNA-SH/Au bioelectrode can specifically detect up to 100- 500 ng/mu L genomic DNA of Vibriocholeare within 60 s of hybridization time at 25 degrees C by cyclic voltammetry (CV) using methylene blue (MB) as electro-active DNA hybridization indicator. The value of sensitivity of the dsDNA/Au electrode has been determined as 0.027 mu A/ng cm(-2) with regression coefficient as 0.978. This DNA bioelectrode is stable for about 4 months when stored at 4 degrees C.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s IOP Publishing
Subjects:	Applied Physics/Condensed Matter Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 11:02
Last Modified:	19 Aug 2026 11:02
URI:	https://npl.csircentral.net/id/eprint/3433

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