Pandey, Chandra Mouli and Sharma, Aditya and Sumana, Gajjala and Tiwari, Ida and Malhotra, Bansi Dhar (2013) Cationic poly(lactic-co-glycolic acid) iron oxide microspheres for nucleic acid detection. Nanoscale, 5 (9). pp. 3800-3807. ISSN 2040-3364

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Abstract

Herein, we envisage the possibility of preparing stable cationic poly(lactic-co-glycolic acid) (PLGA) microspheres encapsulating the iron oxide nanoparticles (IONPs; 8-12 nm). The IONPs are incorporated into PLGA in organic phase followed by microsphere formation and chitosan coating in aqueous medium via nano-emulsion technique. The average size of the microspheres, as determined by dynamic light scattering are about 310 nm, while the zeta potential for the composite remains near 35 mV at pH 4.0. These microspheres are electrophoretically deposited onto indium tin oxide (ITO)-coated glass substrate used as cathode and parallel platinum plate as the counter electrode. This platform is utilized to fabricate a DNA biosensor, by immobilizing a probe sequence specific to Escherichia coli. The bioelectrode shows a surface-controlled electrode reaction with the electron transfer coefficient (alpha) of 0.64 and charge transfer rate constant (k(s)) of 61.73 s(-1). Under the optimal conditions, this biosensor shows a detection limit of 8.7 x 10(-14) M and is found to retain about 81% of the initial activity after 9 cycles of use.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Royal Society of Chemistry
Subjects:	Chemistry Materials Science Applied Physics/Condensed Matter Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 10:40
Last Modified:	19 Aug 2026 10:40
URI:	https://npl.csircentral.net/id/eprint/3322

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