Srivastava, Saurabh and Kumar, Vinod and Ali, Md Azahar and Solanki, Pratima R. and Srivastava, Anchal and Sumana, Gajjala and Saxena, Preeti Suman and Joshi, Amish G and Malhotra, B. D. (2013) Electrophoretically deposited reduced graphene oxide platform for food toxin detection. Nanoscale, 5 (7). 3043-3051. ISSN 2040-3364

PDF - Published Version Restricted to Registered users only Download (696Kb) | Request a copy

Abstract

Reduced graphene oxide (RGO) due to its excellent electrochemical properties and large surface area, has recently aroused much interest for electrochemical biosensing application. Here, the chemically active RGO has been synthesized and deposited onto an indium tin oxide (ITO) coated glass substrate by the electrophoretic deposition technique. This novel platform has been utilized for covalent attachment of the monoclonal antibodies of aflatoxin B-1 (anti-AFB(1)) for food toxin (AFB(1)) detection. The electron microscopy, X-ray diffraction, and UV-visible studies reveal successful synthesis of reduced graphene oxide while the XPS and FTIR studies suggest its carboxylic functionalized nature. The electrochemical sensing results of the anti-AFB(1)/RGO/ITO based immunoelectrode obtained as a function of aflatoxin concentration show high sensitivity (68 mu A ng(-1) mL cm(-2)) and improved detection limit (0.12 ng mL(-1)). The association constant (k(a)) for antigen-antibody interaction obtained as 5 x 10(-4) ng mL(-1) indicates high affinity of antibodies toward the antigen (AFB(1)).

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Royal Society of Chemistry
Subjects:	Chemistry Materials Science Applied Physics/Condensed Matter Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 10:12
Last Modified:	19 Aug 2026 10:12
URI:	https://npl.csircentral.net/id/eprint/3253

Actions (login required)

View Item