Singh, Anu and Sinsinbar, Gaurav and Choudhary, Meenakshi and Kumar, Veeresh and Pasricha, Renu and Verma, H. N. and Singh, Surinder P. and Arora, Kavita (2013) Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid. Sensors and Actuators B: Chemical, 185. pp. 675-684. ISSN 0925-4005

PDF - Published Version Restricted to Registered users only Download (3385Kb) | Request a copy

Abstract

Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5'-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 degrees C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO)

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Elsevier
Subjects:	Chemistry Electrochemistry Instruments/ Instrumentation
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 09:44
Last Modified:	19 Aug 2026 09:44
URI:	https://npl.csircentral.net/id/eprint/3211

Actions (login required)

View Item