Patel, Manoj Kumar and Ali, Md Azahar and Zafaryab, Md and Agrawal, Ved Varun and Rizvi, M. Moshahid Alam and Ansari, Z. A. and Ansari, S. G. and Malhotra, Bansi D. (2013) Biocompatible nanostructured magnesium oxide-chitosan platform for genosensing application. Biosensors and Bioelectronics, 45. pp. 181-188. ISSN 0956-5663

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Abstract

A novel organic-inorganic platform comprising of chitosan (CH) modified nanostructured magnesium oxide (nanoMgO) has been electrophoretically deposited on the indium-tin-oxide (ITO) substrate. The single stranded probe DNA (ssDNA) sequence of Vibrio cholerae has been covalently functionalized onto CH-nanoMgO/ITO surface. The cytotoxicity assay of nanoMgO particles, examined using human intestinal cell line (INT 407), reveals no significant cytotoxicity at the given doses in the range of 50-350m/mu L. The X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and various microscopic techniques have been employed for the structural and morphological analysis of the fabricated electrodes. The electrochemical response studies of ssDNA and fragmented genomic DNA hybridized electrode (dsGDNA/CH-nanoMgO/ITO) have been carried out using cyclic voltammetry (CV) and differential pulse voltammetly (DPV) techniques. The dsGDNA/CH-nanoMgO/ITO bioelectrode exhibits a linear response in the range 100-500 ng/mu L. with improved sensitivity of 36.72 nA/ng/cm(2), faster response time of 3 s and high stability of 3-4 months under refrigerated condition. The lower detection limit of fabricated genosensor has been estimated as 35.20 ng/mu L and it shows good reproducibility/repeatability.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Elsevier
Subjects:	Biophysics Biotechnology & Applied Microbiology Chemistry > Analytical Chemistry Electrochemistry Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 10:41
Last Modified:	19 Aug 2026 10:41
URI:	https://npl.csircentral.net/id/eprint/3330

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