Singh, Renu and Sumana, G. and Verma, Rachna and Sood, Seema and Pandey, M. K. and Gupta, Rajinder K. and Malhotra, B. D. (2010) DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease. Journal of Biotechnology, 150 (3). pp. 357-365. ISSN 0168-1656

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Abstract

A sequence-specific electrochemical sexually transmitted disease (STD) sensor based on self-assembled monolayer of thiolated DNA probe specific to target opa gene for detection of Gonorrhoea, a sexually transmitted disease has been fabricated. 6-Mercapto-1-hexanol (MCH) has been used as a blocking agent to facilitate oligos “stand” up at the surface, a configuration favoring subsequent DNA hybridization and to repel non-specific adsorption of undesired DNA. The results of differential pulse voltammetric studies of this STD sensor reveal low detection limit (1.0×10−18 M) and a wide dynamic range (from 1.0×10−6M to 0.5×10−18 M) arising due to the stable hybridization using methylene blue as an electro-active DNA hybridization indicator. The experimental results with genomic DNA, clinical patient sample of Neisseria gonorrhoeae, culture of non-N. gonorrhoeae Neisseria species (NgNS) and gram negative bacteria indicate that the fabricated sensor is specific to this STD.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s. Elsevier B.V.
Uncontrolled Keywords:	Sexually transmitted disease; Neisseria gonorrhoeae; DNA biosensor; Gold electrode; Methylene blue; Electrochemical sensor
Subjects:	Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Mathematics Medical Laboratory Technology
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	04 Apr 2012 06:14
Last Modified:	04 Apr 2012 06:14
URI:	http://npl.csircentral.net/id/eprint/88

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