Pandey, Chandra Mouli and Singh, Renu and Sumana, Gajjala and Pandey, M. K. and Malhotra, B. D. (2010) Electrochemical genosensor based on modified octadecanethiol self-assembled monolayer for Escherichia coli detection. Sensors and Actuators B: Chemical, 151 (2). pp. 333-340. ISSN 0925-4005

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Abstract

An electrochemical genosensor based on 1-fluoro-2-nitro-4-azidobenzene (FNAB) modified octadecanethiol (ODT) self-assembled monolayer (SAM) has been fabricated for Escherichia coli detection. The results of electrochemical response measurements investigated using methylene blue (MB) as a redox indicator reveal that this nucleic acid sensor has 60 s of response time, high sensitivity (0.5 × 10−18 M) and linearity as 0.5 × 10−18–1 × 10−6 M. The sensor has been found to be stable for about four months and can be used about ten times. It is shown that water borne pathogens like Klebsiella pneumonia, Salmonella typhimurium and other gram-negative bacterial samples has no significant effects in the response of this sensor.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Biosensor; Electrochemical genosensor; Escherichia coli; Octadecanethiol; Self-assembled monolayer
Subjects:	Chemistry > Analytical Chemistry Electrochemistry Instruments/ Instrumentation
Divisions:	UNSPECIFIED
Depositing User:	Mr. Abhishek Yadav
Date Deposited:	18 Dec 2012 07:42
Last Modified:	18 Dec 2012 07:42
URI:	http://npl.csircentral.net/id/eprint/877

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