Srivastava, Saurabh and Solanki, Pratima R. and Kaushik, Ajeet and Ali, Md. Azahar and Srivastava, Anchal and Malhotra, B. D. (2011) A self assembled monolayer based microfluidic sensor for urea detection. Nanoscale , 3 (7). pp. 2971-2977. ISSN 2040-3372

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Abstract

Urease (Urs) and glutamate dehydrogenase (GLDH) have been covalently co-immobilized onto a self-assembled monolayer (SAM) comprising of 10-carboxy-1-decanthiol (CDT) via EDC–NHS chemistry deposited onto one of the two patterned gold (Au) electrodes for estimation of urea using poly(dimethylsiloxane) based microfluidic channels (2 cm × 200 μm × 200 μm). The CDT/Au and Urs-GLDH/CDT/Au electrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, contact angle (CA), atomic force microscopy (AFM) and electrochemical cyclic voltammetry (CV) techniques. The electrochemical response measurement of a Urs-GLDH/CDT/Au bioelectrode obtained as a function of urea concentration using CV yield linearity as 10 to 100 mg dl−1, detection limit as 9 mg dl−1 and high sensitivity as 7.5 μA mM−1 cm−2.

Item Type:	Article
Subjects:	Chemistry Materials Science Applied Physics/Condensed Matter Nanoscience/ Nanotechnology Physics
Divisions:	UNSPECIFIED
Depositing User:	Mr. Abhishek Yadav
Date Deposited:	31 Oct 2012 07:15
Last Modified:	31 Oct 2012 07:15
URI:	http://npl.csircentral.net/id/eprint/778

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