Singh, Renu and Prasad, Rachna and Sumana, G. and Arora, Kavita and Sood, Seema and Gupta, R. K. and Malhotra, B. D. (2008) STD sensor based on nucleic acid functionalized nanostructured polyaniline. Biosensors and Bioelectronics, 24 (7). pp. 2232-2238. ISSN 0956-5663

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Abstract

STD (sexually transmitted disease, Gonorrhoea) sensor based on nucleic acid probe (from Opa, a multi-copy gene of Neisseria gonorrhoeae) functionalized nanostructured-polyaniline coated onto indium–tin-oxide-coated glass plate has been fabricated using avidin–biotin as cross-linking agent. This DNA functionalized electrode can specifically detect upto 0.5 × 10−15 M of complementary target within 60 s of hybridization time at 25 °C by differential pulse voltammetry (DPV) using methylene blue as electro-active DNA hybridization indicator. This highly sensitive and specific nucleic acid functionalized nanostructured-polyaniline electrode can distinguish presence of N. gonorrhoeae from Neisseria meningitidis and Escherichia coli culture and spiked samples from the urethral swabs of the patients.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Sexually transmitted disease; Neisseria gonorrhoeae; DNA biosensor; Polyaniline; Methylene blue; Electrochemical characterization
Subjects:	Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	04 Oct 2012 12:09
Last Modified:	04 Oct 2012 12:09
URI:	http://npl.csircentral.net/id/eprint/655

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