Ansari, Anees A. and Sumana, G. and Khan, R. and Malhotra, B. D. (2009) Polyaniline-Cerium Oxide Nanocomposite for Hydrogen Peroxide Sensor. Journal of Nanoscience and Nanotechnology, 9 (8). pp. 4679-4685. ISSN 1533-4880

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Abstract

Hydrogen peroxide (H2O2) sensor has been fabricated by immobilizing horse radish peroxidase (HRP) onto polyaniline(PANI)-cerium oxide (CeO2) nano-composite film onto indium-tin-oxide (ITO) coated glass substrate using electrochemical technique. The HRP/PANI-CeO2/ITO bioelectrode has been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy, UV-visible spectroscopy and electrochemical analysis, respectively. This electrode shows improved sensitivity (159.6 nA/mM) and detection limit (50 mM). Further this H2O2 sensor shows high HRP affinity to the PANI-CeO2 matrix as indicated by low value (2.9) mM of Michaelis-Menten constant (Km). This bioelectrode exhibits maximum response current at pH 7.0 and retains 90% of its initial response current after about 8 weeks.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s American Scientific Publishers.
Subjects:	Chemistry Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	25 Sep 2012 07:14
Last Modified:	25 Sep 2012 07:14
URI:	http://npl.csircentral.net/id/eprint/606

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