Dhand, Chetna and Solanki, Pratima. R. and Sood, K. N. and Datta, Monika and Malhotra, B. D. (2009) Polyaniline nanotubes for impedimetric triglyceride detection. Electrochemistry Communications , 11 (7). pp. 1482-1486. ISSN 1388-2481

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Abstract

Polyaniline nanotubes (PANI-NT) based film electrophoretically deposited onto indium–tin–oxide (ITO) coated glass plate has been utilized for covalent immobilization of lipase (LIP), via glutaraldehyde (Glu), for triglyceride detection using impedimetric technique. It is shown that fatty acid molecules produced during triglyceride hydrolysis result in change in charge transfer resistance (RCT) of PANI-NT film with varying triglyceride concentration. LIP/Glu/PANI-NT/ITO bioelectrode has linearity as 25–300 mg dL−1, sensitivity as 2.59 × 10−3 KΩ−1 mg−1 dL, response time as 20 s and regression coefficient as 0.99. A low value of apparent Michaelis–Menten constant (∼0.62 mM) indicates high enzyme affinity to tributyrin. The LIP/Glu/PANI-NT/ITO bioelectrode has been utilized to estimate triglyceride in serum samples.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Polyaniline nanotube; Lipase; Triglyceride; Tributyrin; Impedimetric biosensor
Subjects:	Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	24 Sep 2012 12:19
Last Modified:	24 Sep 2012 12:19
URI:	http://npl.csircentral.net/id/eprint/605

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