Kaushik, Ajeet and Solanki, Pratima Rathee and Ansari, Anees Ahmad and Ahmad, Sharif and Malhotra, Bansi Dhar (2008) A nanostructured cerium oxide film-based immunosensor for mycotoxin detection. Nanotechnology , 20 (5). 055105. ISSN 1361-6528

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Abstract

Rabbit-immunoglobulin antibodies (r-IgGs) and bovine serum albumin (BSA) have been immobilized onto sol–gel-derived nanostructured cerium oxide (nanoCeO2) film fabricated onto an indium–tin-oxide (ITO) coated glass plate to detect ochratoxin-A (OTA). Broad reflection planes obtained in x-ray diffraction (XRD) patterns reveal the formation of CeO2 nanostructures. Electrochemical studies reveal that nanoCeO2 particles provide an increased electroactive surface area for loading of r-IgGs with desired orientation, resulting in enhanced electron communication between r-IgGs and electrode. BSA/r-IgGs/nano CeO2/ITO immunoelectrode exhibits improved characteristics such as linear range (0.5–6 ng dl−1), low detection limit (0.25 ng dl−1), fast response time (30 s) and high sensitivity (1.27 µA ng−1 dl−1 cm−2). The high value of the association constant (Ka, 0.9 × 1011 l mol−1) indicates the high affinity of the BSA/r-IgGs/nanoCeO2/ITO immunoelectrode to OTA.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Institute of Physics.
Subjects: Materials Science
Physics
Divisions: UNSPECIFIED
Depositing User: Ms Neetu Chandra
Date Deposited: 19 Sep 2012 12:14
Last Modified: 19 Sep 2012 12:14
URI: http://npl.csircentral.net/id/eprint/576

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