Solanki, Pratima R. and Dhand, Chetna and Kaushik, Ajeet and Ansari, Anees A. and Sood, K. N. and Malhotra, B. D. (2009) Nanostructured cerium oxide film for triglyceride sensor. Sensors and Actuators B: Chemical, 141 (2). 551-556 . ISSN 0925-4005

PDF - Published Version Restricted to Registered users only Download (814Kb) | Request a copy

Abstract

Lipase has been immobilized onto sol–gel derived nanostuctured cerium oxide (Nano-CeO2, 35 nm) film deposited onto indium–tin–oxide (ITO) coated glass plate for tributyrin detection. The Nano-CeO2/ITO electrode and lipase/nano-CeO2/ITO bioelectrode have been characterized using scanning electron microscopy (SEM) and cyclic voltammetry (CV). The electrochemical response of lipase/nano-CeO2/ITO bioelectrode towards tributyrin, investigated using CV studies, exhibits linearity, detection limit and shelf life as 50–500 mg/dL, 32.8 mg/dL and 12 weeks, respectively. The value of the apparent Michaelis–Menten constant (View the MathML source) obtained as 22.27 mg/dL (0.736 mM) for lipase/nano-CeO2/ITO bioelectrode indicates high affinity of lipase with tributyrin. Attempts have been made to detect triglyceride concentration in serum samples.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Nanostructured cerium oxide; Lipase; Triglyceride; Tributyrin; Electrochemical biosensor
Subjects:	Chemistry Electrochemistry Instruments/ Instrumentation
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	19 Sep 2012 11:35
Last Modified:	19 Sep 2012 11:35
URI:	http://npl.csircentral.net/id/eprint/575

Actions (login required)

View Item