Matharu, Zimple and Arya, Sunil K. and Singh, S. P. and Gupta, Vinay and Malhotra, B. D. (2008) Langmuir–Blodgett film based on MEH-PPV for cholesterol biosensor. Analytica Chimica Acta , 634 (2). pp. 243-249. ISSN 0003-2670

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Abstract

Cholesterol oxidase (ChOx) has been immobilized onto conducting poly[2-methoxy,5-(2′-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV)/stearic acid (SA) Langmuir–Blodgett film transferred onto octadecanethiol (ODT) modified gold plate. The ChOx/MEH-PPV/SA LB film bioelectrode exhibits has been characterized by FT-IR, contact angle, and atomic force microscopy. The response of the ChOx/MEH-PPV/SA LB film bioelectrode carried out using differential pulse voltammetry (DPV) studies reveal linearity from 1.29 to 12.91 mM of cholesterol concentration and response time as 30 s. This ChOx/MEH-PPV/SA bioelectrode exhibits values of correlation coefficient as 0.9939, standard deviation as 0.0029 μA and limit of detection as 1.66 mM. UV–visible spectrophotometer studies reveal that 5.2 × 10−3 U of ChOx are actively working per cm2 area of ChOx/MEH-PPV/SA LB film bioelectrode and this bioelectrode is thermally stable upto 55 °C with reusability of about 60 times.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Langmuir–Blodgett film; poly[2-methoxy,5-(2′-ethyl-hexyloxy)-1,4-phenylene vinylene]; Cholesterol oxidase; Differential pulse voltammetry; Ultraviolet–visible
Subjects:	Chemistry Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	10 Sep 2012 06:53
Last Modified:	10 Sep 2012 06:53
URI:	http://npl.csircentral.net/id/eprint/552

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