Kaushik, Ajeet and Solanki, Pratima R. and Ansari, Anees A. and Malhotra, Bansi D. and Ahmad, Sharif (2009) Iron oxide-chitosan hybrid nanobiocomposite based nucleic acid sensor for pyrethroid detection. Biochemical Engineering Journal , 46 (2). pp. 132-140. ISSN 1369-703X

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Abstract

Nucleic acid sensor has been fabricated via immobilization of single standard calf thymus deoxyribose nucleic acid (ssCT-DNA) onto chitosan (CH)-iron oxide (Fe3O4) nanoparticles based hybrid nanobiocomposite film deposited onto indium-tin-oxide (ITO) coated glass for pyrethroids [cypermehtirn (CM) and permethrin (PM)] detection. The ssCT-DNA immobilized onto CH-Fe3O4 nanocomposite/ITO electrode has been characterized using scanning electron microscopy (SEM), UV–visible, Fourier transform infrared (FTIR) spectroscopy, electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) techniques. This disposable ssCT-DNA/CH-Fe3O4 nanobiocomposite/ITO bioelectrode is stable for about two months under refrigerated conditions and can detect CM (0.0025–2 ppm) and PM (1–300 ppm) using DPV technique within 25 s and 40 s, respectively.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Biosensor; Biocatalysis; Biopolymers; Fe3O4 nanoparticles; Nanobiocomposite; Electrochemical detection
Subjects:	Biotechnology & Applied Microbiology Engineering
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	07 Sep 2012 12:33
Last Modified:	07 Sep 2012 12:33
URI:	http://npl.csircentral.net/id/eprint/549

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