Ansari, Anees A. and Solanki, Pratima R. and Malhotra, B. D. (2009) Hydrogen peroxide sensor based on horseradish peroxidase immobilized nanostructured cerium oxide film. Journal of Biotechnology, 142 (2). pp. 179-184. ISSN 0168-1656

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Abstract

Nanostructured cerium oxide (NanoCeO2) film deposited onto indium-tin-oxide (ITO) glass substrate by solution casting has been used for immobilization of horseradish peroxidase (HRP) via physiosorption technique. X-ray diffraction (XRD), scanning electron microscopy (SEM), UV–vis and electrochemical techniques have been utilized for characterization of NanoCeO2/ITO electrode and HRP/NanoCeO2/ITO bioelectrode. The HRP/NanoCeO2/ITO electrode exhibits value of the apparent Michaelis–Menten constant (Km) as 2.21 μM, linear regression coefficient as 0.998 and linearity for hydrogen peroxide as 1.0–170 μM obtained using electrochemical response measurements. Besides this, HRP/NanoCeO2/ITO bioelectrode can be used about 20 times and is stable for 5 weeks at 4 °C. The results of photo-response studies carried out on HRP/NanoCeO2/ITO bioelectrode indicate reasonable agreement with those obtained using amperometric technique.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Horseradish peroxidase (HRP); NanoCeO2; Biosensor; Hydrogen peroxide
Subjects:	Biotechnology & Applied Microbiology
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	03 Sep 2012 08:07
Last Modified:	03 Sep 2012 08:07
URI:	http://npl.csircentral.net/id/eprint/526

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