Khan, Raju and Solanki, Pratima R. and Kaushik, Ajeet and Singh, S. P. and Ahmad, Sharif and Malhotra, B. D. (2008) Cholesterol biosensor based on electrochemically prepared polyaniline conducting polymer film in presence of a nonionic surfactant. Journal of Polymer Research , 16 (4). pp. 363-373. ISSN 1572-8935

PDF - Published Version Restricted to Registered users only Download (408Kb) | Request a copy

Abstract

Cholesterol biosensor has been fabricated by covalently coupling cholesterol oxidase (ChOx) via glutaraldehyde onto electrochemically prepared polyaniline film in presence of TritonX-100 [4-(1,1,3,3-tetramethylbutyl) phenyl polyethylene glycol], a non-ionic surfactant onto indium-tin-oxide (ITO) glass substrate. These ChOx/PANI-TX-100/ITO bioelectrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV) and scanning electron microscopy (SEM) techniques. The results of response measurements carried out on ChOx/PANI-TX-100/ITO bioelectrodes using amperometric and photometric techniques, reveal detection limit as 5 mg/dl, linearity from 5 to 400 mg/dl of cholesterol and sensitivity as 131 μA/(mg/dl cm−2). These biosensing electrodes are thermally stable up to 65 °C, can be used about 20 times and have a shelf-life of about 10 weeks when stored at 4 °C. Attempts have also been made to utilize the ChOx/PANI-TX-100/ITO bioelectrodes for estimation of free cholesterol concentration in serum samples.

Item Type:	Article
Additional Information:	Copyright for this article belongs to Springer Science+Business Media.
Subjects:	Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	16 Aug 2012 07:57
Last Modified:	16 Aug 2012 07:57
URI:	http://npl.csircentral.net/id/eprint/425

Actions (login required)

View Item