Tiwari, A. and Shukla, S. K. (2009) Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor. Express Polymer Letters, 3 (9). pp. 553-559. ISSN 1788-618X

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Abstract

A novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-g-PANI biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), contact angle measurement and cyclic voltammetry (CV) taking CHIT-g-PANI as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg/cm2, respectively; indicating CHIT-g-PANI matrix has a high affinity to immobilize CAH enzyme.

Item Type:	Article
Subjects:	Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	16 Aug 2012 07:32
Last Modified:	16 Aug 2012 07:32
URI:	http://npl.csircentral.net/id/eprint/423

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