Bhardwaj, Hema and Pandey, Manoj Kumar and -, Rajesh and Sumana, Gajjala (2019) Electrochemical Aflatoxin B1 immunosensor based on the use of graphene quantum dots and gold nanoparticles. Microchimica Acta, 186 (8). pp. 592-603. ISSN 0026-3672

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Abstract

Electrochemical immunosensor for aflatoxin B1 (AFB1) is described that uses a composite prepared from graphene quantum dots (GQDs) and gold nanoparticles (Au NPs). The GQD-AuNP conjugate was obtained by using 2-aminothiophenol (ATP) as a linker where the carboxy groups of GQD bind to the amino groups of crosslinker via conjugation of thiol binding to the AuNP. To evaluate the conjugation of the GQD-AuNP composite, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM) was applied. The composite was placed on an indium tin oxide (ITO) electrode and then modified with an antibody against AFB1. By using hexacyanoferrate as the electrochemical probe, the sensor works in the 0.1 to 3.0ngmL(-1) AFB1 concentration range, is highly specific, has good reproducibility and acceptable stability. The biosensor was applied to the analysis of (spiked) maize samples. Conceivably, the method can be utilized to sense other mycotoxins by using their respective antibodies.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Springer Verlag.
Subjects:	Chemistry > Analytical Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Mr. Yogesh Joshi
Date Deposited:	02 Mar 2020 07:32
Last Modified:	02 Mar 2020 07:32
URI:	http://npl.csircentral.net/id/eprint/4169

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