Kaushik, Ajeet and Solanki, Pratima R. and Pandey, M. K. and Ahmad, Sharif and Malhotra, Bansi D.
(2009)
Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection.
Applied Physics Letters, 95 (17).
173703 -1.
ISSN 1077-3118
Abstract
Cerium oxide nanoparticles (NanoCeO2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO2 increases effective electro-active surface area of CH-NanoCeO2/indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA/r-IgGs/CH-NanoCeO2/ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μA/ng dl−1 cm−2), and regression coefficient (r2 ∼ 0.997).
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