Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection

Das, Maumita and Sumana, Gajjala and R. , Nagarajan and Malhotra, B. D. (2010) Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection. Applied Physics Letters, 96 (13). 133703-1-133703-3. ISSN 1077-3118

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Official URL: http://dx.doi.org/10.1063/1.3293447

Abstract

Nanostructured zirconium oxide (ZrO2) film (particle size ∼ 35 nm), electrochemically deposited onto gold(Au) surface, has been used to immobilize 21-mer oligonucleotide probe (ssDNA) specific to Mycobacterium tuberculosis by utilizing affinity between oxygen atom of phosphoric group and zirconium to fabricate DNA biosensor. This DNA-ZrO2/Au bioelectrode, characterized using x-ray diffraction, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy techniques, can be used for early and rapid diagnosis of M. tuberculosis with detection limit of 0.065 ng/μL within 60s.

Item Type:	Article
Subjects:	Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	08 Aug 2012 11:50
Last Modified:	08 Aug 2012 11:50
URI:	http://npl.csircentral.net/id/eprint/395

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