Kumar, Sumit and Kumar, Jitendra and Sharma, Shailesh Narayan (2018) Nanostructured titania based electrochemical impedimetric biosensor for non-invasive cancer detection. Materials Research Express, 5 (12). pp. 125405-125418. ISSN 2053-1591

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Abstract

In the present work, the utilisation of bio-functionalised TiO2 nanoparticles has been reported for biosensing application especially in oral cancer detection. The bio-sensing electrode was fabricated on indium tin oxide coated conducting glass electrode using electrophoretic deposition technique. The proposed technique holds tremendous potential to make the approach effectively simple, label-free and most importantly a non-invasive technique. The prepared nanoparticles were characterised using x-ray diffractometer; Fourier transforms infrared spectroscopy, and x-ray photoelectron spectroscopy. In conjunction with this, the morphological studies of immunoelectrode (bovine serum albumin/anti-CYFRA-21-1/(3-aminopropyl) triethoxysilane/TiO2/indium tin oxide) were conducted by field emission scanning electron microscopy. The average roughness of various electrodes were investigated through atomic force microscopy. The biosensing properties of fabricated immunoelectrode were investigated using differential pulse voltammetry and cyclic voltammetry. However, the electrochemical response studies were carried out by using electrochemical impedance spectroscopy technique to measure the concentration of oral cancer biomarker (CYFRA 21-1). Additionally, we have also calculated the sensitivity and stability of fabricated immunoelectrode. It has been observed that the fabricated immunoelectrode shows a high sensitivity of 0.573 Omega mL ng(-1), linear detection range of 0-12 ng mL(-1), lower detection limit of 0.24 ng mL(-1) and having a stability of 5 weeks.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s IOP Publishing.
Subjects:	Materials Science
Divisions:	UNSPECIFIED
Depositing User:	Mr. Yogesh Joshi
Date Deposited:	07 Nov 2019 11:43
Last Modified:	07 Nov 2019 11:43
URI:	http://npl.csircentral.net/id/eprint/3927

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