Patel, Manoj Kumar and Ali, Md. Azahar and Srivastava, Saurabh and Agrawal, Ved Varun and Ansari, S. G. and Malhotra, Bansi D. (2013) Magnesium oxide grafted carbon nanotubes based impedimetric genosensor for biomedical application. Biosensors and Bioelectronics, 50. pp. 406-413. ISSN 0956-5663

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Abstract

Nanostructured magnesium oxide (size < 10 nm) grafted carboxyl (COOH) functionalized multi-walled carbon nanotubes (nMgo-cMWCNTs) deposited electrophoretically onto indium tin oxide (ITO) coated glass electrode and have been utilized for Vibrio cholerae detection. Aminated 23 bases single stranded DNA (NH2 ssDNA) probe sequence (01 gene) of V cholerae has been covalently functionalized onto nMgo-cMWCNTs/ITO electrode surface using EDC-NHS chemistry. This DNA functionalized MgO grafted cMWCNTs electrode has been characterized using X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and electrochemical techniques. The results of XPS studies reveal that sufficient 0 C=O groups present at the nMgo-cMWCNTs surface are utilized for DNA binding. The results of hybridization studies conducted with fragmented target DNA (ftDNA) of V cholerae using electrochemical impedance spectroscopy (EIS) reveal sensitivity as 3.87 52 ng(-1) cm(-2), detection limit of similar to 21.70 ng mu L-1 in the linear range of 100-500 ng mu L-1 and stability of about 120 days. The proposed DNA functionalized nMgO-cMWCNTs nanomatrix provides a novel impedimetric platform for the fabrication of a compact genosensor device for biomedical application.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier.
Subjects:	Biophysics Biotechnology & Applied Microbiology Chemistry > Analytical Chemistry Electrochemistry Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	09 Jul 2020 11:03
Last Modified:	09 Jul 2020 11:03
URI:	http://npl.csircentral.net/id/eprint/3141

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