Srivastava, Saurabh and Ali, Md. Azahar and Solanki, Pratima R. and Chavhan, Pandurang M. and Pandey, Manoj K. and Mulchandani, Ashok and Srivastava, Anchal and Malhotra, Bansi D.
(2013)
Mediator-free microfluidics biosensor based on titania-zirconia nanocomposite for urea detection.
RSC Advances , 3 (1).
pp. 228-235.
ISSN 2046-2069
Abstract
Urease (Urs) and glutamate dehydrogenase (GLDH) co-immobilized onto titania-zirconia (TiO2-ZrO2) nanocomposite and integrated with microfluidics mediator-free sensor have been utilized for urea detection. The PDMS microchannels have been sealed with a glass substrate comprising of reference (Ag/AgCl), counter (ITO) and working (Urs-GLDH/TiO2-ZrO2/ITO) electrodes. This mediator-free microfluidics urea sensor shows linearity as 5-100 mg/dL with improved sensitivity as 2.74 mu A [Log mM](-1) cm(-2) and detection limit of 0.07 mg/dl (0.44 mM) using 3 sigma(b)/m criteria. The Reynolds number has been found to be as 0.166, indicating that fluid flow is completely laminar, controllable and the pressure drop across the microchannels is found to be as 3.5 x 10(3) Pa.
| Item Type: |
Article
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| Additional Information: |
Copyright for this article belongs to M/s Royal Society of Chemistry. |
| Subjects: |
Chemistry |
| Divisions: |
UNSPECIFIED |
| Depositing User: |
Users 27 not found. |
| Date Deposited: |
09 Jul 2020 10:26 |
| Last Modified: |
09 Jul 2020 10:26 |
| URI: |
http://npl.csircentral.net/id/eprint/3128 |
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