Charak, Sonika and Mehrotra, Ranjana (2013) Structural investigation of idarubicin-DNA interaction: Spectroscopic and molecular docking study. International Journal of Biological Macromolecules, 60. pp. 213-218. ISSN 0141-8130

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Abstract

Mechanistic understanding of interaction of drugs with their target molecule is important for development of new drug therapy regimes. Idarubicin (IDR) is a potent chemotherapeutic agent used to treat variety of cancers. Structural and conformational studies associated with binding of IDR on DNA double helix were investigated through spectroscopic techniques and molecular docking studies. Interaction studies were done by preparing different molar ratios of IDR with constant DNA concentration under physiological conditions. FTIR spectroscopy, UV-vis spectroscopy, CD spectroscopy were used to analyze interaction between IDR and DNA. FUR results suggest IDR binds at major groove of DNA duplex via guanine and cytosine bases. UV-vis spectroscopy result indicates IDR gets intercalated between the DNA bases. The calculated binding constant shows that IDR is a moderate binder. Slight perturbation in the native B-conformation of DNA was observed in all IDR-DNA molar ratios examined. In silico investigation of IDR binding with DNA is in agreement with our experimental results, providing structural insight into DNA binding properties of IDR.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier.
Subjects:	Biochemistry & Molecular Biology Chemistry > Polymer Science Chemistry > Applied Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	09 Jun 2020 10:06
Last Modified:	09 Jun 2020 10:06
URI:	http://npl.csircentral.net/id/eprint/3040

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