Pandey, Chandra Mouli and Tiwari, Ida and Singh, Vidya Nand and Sood, K. N. (2017) Highly sensitive electrochemical immunosensor based on graphene-wrapped copper oxide-cysteine hierarchical structure for detection of pathogenic bacteria. Sensors and Actuators B: Chemical, 238. pp. 1060-1069. ISSN 0925-4005

PDF - Published Version Restricted to Registered users only Download (3494Kb) | Request a copy

Abstract

We report results of the studies relating to fabrication of the graphene wrapped copper (II) assisted cysteine hierarchical structure (rGO-CysCu, 10 mu m) synthesised using facile, aqueous and environmental friendly conditions. The results of electrochemical impedance spectroscopic investigations indicate that self-assembly of rGO-CysCu molecules onto gold electrode provides a high surface area and high electron transfer rate constant (1.82 x 10(-6) cm/s). Further, an ultrasensitive label-free electrochemical immunosensor for quantitative determination of Escherichia coli O157: H7 (E. coli) has been developed using rGO-CysCu as the sensing layer. Under optimal conditions, the calibration plot pertaining to sensing characteristics of the fabricated immunoelectrode for E. coli O157: H7 was approximately linear in the wide detection range of 10 CFUmL(-1) to 10(8) CFUmL(-1) with a detection limit of 3.8 CFUmL(-1). Moreover, the proposed method was successfully used to differentiate the E. coli O157: H7 cells from the non-pathogenic E. coli (DH5 alpha) and other bacterial cells in the synthetic samples.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier.
Subjects:	Chemistry > Analytical Chemistry Electrochemistry Instruments/ Instrumentation
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	21 May 2020 11:28
Last Modified:	21 May 2020 11:28
URI:	http://npl.csircentral.net/id/eprint/2945

Actions (login required)

View Item