Saha, Shibu and Arya, Sunil K. and Singh, S. P. and K. , Sreenivas and Malhotra, B. D. and Gupta, Vinay (2010) PLD grown ZnO–K3[Fe(CN)6] composite thin film for biosensing application. Thin Solid Films, 519 (3). pp. 1184-1186. ISSN 0040-6090

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Abstract

Zinc oxide–potassium ferricyanide (ZnO–KFCN) composite film was prepared on ITO coated corning glass using pulsed laser deposition (PLD). The composite film has proved to be a suitable platform for enzyme immobilization. The composite matrix exhibits the advantages of ZnO along with enhanced redox property due to the presence of a mediator in the matrix. Glucose oxidase (GOx) has been chosen as the model enzyme for studying the application of the present matrix to biosensing. The sensing response of the bio-electrode, GOx/ZnO–KFCN/ITO/glass, towards glucose was studied by electrochemical and photometric techniques. The bio-electrode exhibits good linearity and low value of Michaelis–Menten constant. Due to efficient biosensing in a mediator-less system the present bio-electrode should lead to a hand held integrated lab-on-chip device.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Subjects:	Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	18 Jun 2012 10:35
Last Modified:	18 Jun 2012 10:35
URI:	http://npl.csircentral.net/id/eprint/276

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