Prabhakar, Nirmal and Solanki, Pratima R. and Kaushik, Ajeet and Pandey, M. K. and Malhotra, B. D. (2010) Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection. Electroanalysis , 22 (22). pp. 2672-2682. ISSN 1521-4109

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Abstract

We have prepared nanocomposite films comprising of 3-glycidoxypropyltrimethoxysilane (GOPS) and iron-oxide (Fe3O4) onto indium-tin-oxide (ITO) glass plate for covalent immobilization of 21-mer peptide nucleic acid (PNA). These films have been characterized using contact angle, atomic force microscopy (AFM), electrochemical techniques. The electrochemical response of the GOPS/ITO and Fe3O4-GOPS/ITO electrodes has been investigated by hybridization with complementary, non-complementary and one-base mismatch using methylene blue as electrochemical indicator. The PNA/Fe3O4-GOPS/ITO bioelectrode exhibits improved specificity and detection limit (0.1 fM) as compared to that of the PNA-GOPS/ITO bioelectrode (0.1 pM). This PNA/Fe3O4-GOPS/ITO electrode can be utilized for detection of hybridization with the complementary sequence in sonicated M. tuberculosis genomic DNA within 90 s of hybridization time.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Wiley-VCH Verlag Berlin
Subjects:	Chemistry Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	18 Jun 2012 09:46
Last Modified:	18 Jun 2012 09:46
URI:	http://npl.csircentral.net/id/eprint/274

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