Ansari, Anees A. and Kaushik, Ajeet and Solanki, Pratima R. and Malhotra, B. D. (2009) Nanostructured zinc oxide platform for mycotoxin detection. Bioelectrochemistry , 77 (2). pp. 75-81. ISSN 1878-562X

PDF - Published Version Restricted to Registered users only Download (1198Kb) | Request a copy

Abstract

Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium–tin–oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as ~5.0 nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006–0.01 nM/dm3, detection limit of 0.006 nM/dm3, response time as 25 s and sensitivity of 189 Ω/nM/dm3cm− 2 with a regression coefficient of 0.997.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Sol–gel; Zinc oxide; Impedimetric immunosensor; Mycotoxin; Ochratoxin-A;
Subjects:	Biochemistry & Molecular Biology Biophysics Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	13 Jun 2012 10:55
Last Modified:	13 Jun 2012 10:55
URI:	http://npl.csircentral.net/id/eprint/256

Actions (login required)

View Item