Arya, S. K. and Solanki, P. R. and Singh, R. P. and Pandey, M. K. and Datta, M. and Malhotra, B. D. (2006) Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon resonance technique. Talanta, 69 (4). pp. 918-926. ISSN 0039-9140

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Abstract

Octadecanethiol (ODT) self-assembled monolayer (SAM) prepared onto gold-coated glass plate has been modified by using nitrene reaction of 1-fluoro-2-nitro-4-azidobenzene (FNAB) that further covalently binds to cholesterol oxidase (ChOx) via thermal reaction. FNAB acts as a bridge (cross-linker) between SAM and ChOx. The ChOx/FNAB/ODT/Au electrode thus fabricated has been characterized using contact angle (CA) measurements, UV-vis spectroscopy, electrochemical techniques and X-ray photoelectron spectroscopy (XPS) technique, respectively. This ChOx/FNAB/ODT/Au bioelectrode has been utilized for estimation of cholesterol in solution using surface plasmon resonance (SPR) technique. This SPR based cholesterol biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with lower detection limit of 50 mg/dl and shelf life of about 2 months when stored at 4 degrees C.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier.
Subjects:	Chemistry > Analytical Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	07 May 2018 08:33
Last Modified:	07 May 2018 08:33
URI:	http://npl.csircentral.net/id/eprint/2419

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