-, Rajesh and Sharma, Vikash and Puri, Nitin K. and Mulchandani, Ashok
(2016)
High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection.
Applied Physics Letters, 109 (24).
ISSN 0003-6951
Abstract
We report a single-walled carbon nanotube (SWNT) field-effect transistor (FET) functionalized with Polyamidoamine (PAMAM) dendrimer with 128 carboxyl groups as anchors for site specific biomolecular immobilization of protein antibody for C-reactive protein (CRP) detection. The FET device was characterized by scanning electron microscopy and current-gate voltage (I-V-g) characteristic studies. A concentration-dependent decrease in the source-drain current was observed in the regime of clinical significance, with a detection limit of similar to 85 pM and a high sensitivity of 20% change in current (Delta I/I) per decade CRP concentration, showing SWNT being locally gated by the binding of CRP to antibody (anti-CRP) on the FET device. The low value of the dissociation constant (K-d = 0.31 +/- 0.13 mu g ml(-1)) indicated a high affinity of the device towards CRP analyte arising due to high anti-CRP loading with a better probe orientation on the 3-dimensional PAMAM structure.
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