Bhardwaj, Hema and Solanki, Shipra and Sumana, Gajjala (2015) Electrophoretically deposited multiwalled carbon nanotube based amperometric genosensor for E. coli detection. In: India-Japan Expert Group Meeting on Biomolecular Electronics and Organic Nanotechnology for Environment Preservation (IJEGMBE) , DEC 22-25, 2015, Kyushu Inst Technol, Fukuoka, JAPAN .

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Abstract

This work reports on a sensitive and selective genosensor fabrication method for Escherichia coli (E. coli) detection. The functionalized multiwalled carbon nanotubes (MWCNT) synthesized via chemical vapour deposition have been deposited electrophoretically onto indium tin oxide coated glass surface and have been utilized as matrices for the covalent immobilization of E. coli specific probe oligonucleotide that was identified from the 16s rRNA coding region of the E. coli genome. This fabricated functionalized MWCNT based platform sought to provide improved fundamental characteristics to electrode interface in terms of electro-active surface area and diffusion coefficient. Electrochemical cyclic voltammetry revealed that this genosensor exhibits a linear response to complementary DNA in the concentration range of 10(-7) to 10(-12) M with a detection limit of 1x10(-12) M.

Item Type:	Conference or Workshop Item (Paper)
Additional Information:	Book Series: Journal of Physics Conference Series Volume: 704 Published: 2016
Subjects:	Engineering > Electronics and Electrical Engineering Nanoscience/ Nanotechnology Physics
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	17 Nov 2017 11:32
Last Modified:	17 Nov 2017 11:32
URI:	http://npl.csircentral.net/id/eprint/2204

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