Solanki, Shipra and Pandey, Chandra Mouli and Soni, Amrita and Sumana, Gajjala and Biradar, Ashok Manikrao (2016) An amperometric bienzymatic biosensor for the triglyceride tributyrin using an indium tin oxide electrode coated with electrophoretically deposited chitosan-wrapped nanozirconia. Microchimica Acta, 183 (1). 167-176 . ISSN 0026-3672

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Abstract

A nanocomposite was prepared from chitosan and zirconium oxide nanoparticles (nano-ZrO2) and then electrophoretically deposited on an indium tin oxide (ITO) coated glass substrate. Morphological investigations reveal a uniform wrapping of nano-ZrO2 in chitosan, with a particle size of similar to 20 nm. The enzymes lipase and glycerol dehydrogenase were immobilized on this electrode to obtain an amperometric biosensor for the lipid tributyrin. Lipase hydrolyzes tributyrin to form glycerol which is oxidized by glycerol dehydrogenase under the formation of reduced nicotinamide adenine dinucleotide (NADH). The electrochemical response of the NADH formed was studied via cyclic voltammetry using hexacyanoferrate as a mediator. The current measured (best at a working voltage of 0.35 V vs. Ag/AgCl) is related to the concentration of tributyrin in the 0.25 to 4 mg mL(-1) range. The lower limit of detection is 155 A mu g mL(-1), and the response time is 45 s. The sensor is fairly stable in that its activity is reduced to 85% within a period of 11 weeks. This bioelectrode has been applied to the determination of triglycerides in serum samples.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Springer Verlag.
Uncontrolled Keywords:	Enzymatic biosensor Lipase Glycerol dehydrogenase Zirconiumoxide Sol–gel Electrophoretic deposition NADH Triglyceride Hexacyanoferrate Cyclic voltammetry
Subjects:	Chemistry > Analytical Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	02 Jun 2017 08:09
Last Modified:	02 Jun 2017 08:09
URI:	http://npl.csircentral.net/id/eprint/2141

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