Khan, Raju and Kaushik, Ajeet and Solanki, Pratima R. and Ansari, Anees A. and Pandey, Manoj K. and Malhotra, B. D. (2008) Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor. Analytica Chimica Acta , 616 (2). pp. 207-213. ISSN 0003-2670

PDF - Published Version Restricted to Registered users only Download (876Kb) | Request a copy

Abstract

Zinc oxide nanoparticles (NanoZnO) uniformly dispersed in chitosan (CHIT) have been used to fabricate a hybrid nanocomposite film onto indium-tin-oxide (ITO) glass plate. Cholesterol oxidase (ChOx) has been immobilized onto this NanoZnO-CHIT composite film using physiosorption technique. Both NanoZnO-CHIT/ITO electrode and ChOx/NanoZnO-CHIT/ITO bioelectrode have been characterized using Fourier transform-infrared (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) techniques, respectively. The ChOx/NanoZnO-CHIT/ITO bioelectrode exhibits linearity from 5 to 300 mg dl(-1) of cholesterol with detection limit as 5 mg dl(-1), sensitivity as 1.41 x 10(-4) A mg dl(-1) and the value of Michaelis-Menten constant (K-m) as 8.63 mg dl(-1). This cholesterol biosensor can be used to estimate cholesterol in serum samples.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Subjects:	Chemistry Chemistry > Analytical Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	06 Apr 2017 07:06
Last Modified:	06 Apr 2017 07:06
URI:	http://npl.csircentral.net/id/eprint/2088

Actions (login required)

View Item