Matharu, Zimple and Arya, Sunil K. and Sumana, G. and Gupta, Vinay and Malhotra, B. D. (2008) Self-assembled monolayer for low density lipoprotein detection. Journal of Molecular Recognition , 21 (6). 419-424 . ISSN 1099-1352

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Abstract

Heparin (HEP) has been covalently immobilized onto 4-aminothiophenol (ATIP) self-assembled monolayer (SAM) deposited onto gold (Au)-coated glass plate for low density lipoprotein (LDL) detection. The HEP/ATP/Au and LDL/ HEP/ATP/Au electrodes have been characterized using cyclic voltammetry (CV) and scanning electron microscopy (SEM). Surface plasmon resonance (SPIR) measurements reveal that HEP/ATP/Au electrode is sensitive to detection of the LDL in the range 0.03 mu M (10mg/dl)-0.39 mu M (130mg/dl). The values of association and dissociation rate constants in the association phase calculated by kinetic analysis have been found to be k(a) = 9.67 X 10(1) M(-1) s(-1) and k(d) = 2.64 X 10(-4) s(-1).

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s John Wiley and Sons.
Subjects:	Biochemistry & Molecular Biology Biology Biophysics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	05 Apr 2017 12:01
Last Modified:	05 Apr 2017 12:01
URI:	http://npl.csircentral.net/id/eprint/2084

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