Gupta, Sonali and Singh, Surinder P. and Singh, Rajni (2015) Synergistic Effect of Reductase and Keratinase for Facile Synthesis of Protein-Coated Gold Nanoparticles. Journal of Microbiology and Biotechnology, 25 (5). 612-619 . ISSN 1017-7825

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Abstract

We have synthesized gold nanoparticles (GNPs) using chicken feathers (poultry waste) and Bacillus subtilis RSE163. Disulfide reductase and keratirtase produced by Bacillus subtilis during the degradation of chicken feather has been used to reduce Au3+ from HAuCl4 precursor to produce gold nanoparticles. The synthesized biogenic GNPs were characterized by UV-visible spectroscopy, transmission electron microscopy (TEM), and zeta potential measurements. Fourier transform infrared (FTIR) spectroscopy indicated the presence of protein capping on synthesized GNPs, imparting multifunctionality to the GNP surface. Furthermore, the nontoxic nature of biogenic GNPs was insured by interaction with Escherichia coli (ATCC11103), where TEM images and enhancement of growth rate of E. coli in log phase signified their nontoxic nature. The results indicate that the synthesis of biocompatible GNPs using poultry waste may find potential applications in drug delivery and sensing.

Item Type:	Article
Uncontrolled Keywords:	Reductase, keratinase, gold nanoparticles, UV-visible spectroscopy, bioreduction
Subjects:	Biotechnology & Applied Microbiology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	21 Oct 2016 05:32
Last Modified:	21 Oct 2016 05:32
URI:	http://npl.csircentral.net/id/eprint/2023

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