Sharma, Parul and Mathur, Garima and Goswami, Navendu and Sharma, Sanjeev K. and Dhakate, Sanjay R. and Chand, Subhash and Mathur, Ashwani (2015) Evaluating the potential of chitosan/poly(vinyl alcohol) membranes as alternative carrier material for proliferation of Vero cells. E-polymers, 15 (4). 237-243. ISSN 1618-7229

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Abstract

Chitosan/poly(vinyl alcohol) (CS/PVA) blend membranes were prepared using the casting method and their physiochemical properties were analyzed using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and X-ray diffraction (XRD). FTIR and XRD demonstrated possible hydrogen bonds between CS and PVA. The addition of PVA to CS resulted in surface roughness as analyzed by SEM. The CS/PVA blend membrane exhibited high tensile properties (81.62%) and reduced water-holding capacity (53.8%) compared to a pure CS membrane (control). Cell viability and proliferation were assessed via an MTT assay with Vero cell culture. Associated with improved physicochemical properties, the CS/PVA blend membrane promotes cell proliferation of Vero cells with high specific growth rate (0.582 day(-1)). The results demonstrate that the blending of CS and PVA could significantly alter the surface rugosity, water-holding capacity and improve the mechanical and biological properties of the membrane. Interestingly, this concept can be extended for different anchorage-dependent cell lines, as an alternative carrier material.

Item Type:	Article
Uncontrolled Keywords:	cell culture; chitosan; DSC; poly(vinyl alcohol)
Subjects:	Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	23 Sep 2016 05:05
Last Modified:	23 Sep 2016 05:05
URI:	http://npl.csircentral.net/id/eprint/1812

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