Patel, Manoj Kumar and Md. Azahar Ali, Md. Azahar and Krishnan, Sadagopan and Agrawal, Ved Varun and Kheraif, AbdulAziz A. Al and Fouad, H. and Ansari, Z.A. and Ansari, S. G. and Malhotra, Bansi D. (2015) A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection. SCIENTIFIC REPORTS , 5 (17384). 1 -8. ISSN 2045-2322

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Abstract

Nanomaterial-based photoluminescence (PL) diagnostic devices offer fast and highly sensitive detection of pesticides, DNA, and toxic agents. Here we report a label-free PL genosensor for sensitive detection of Vibrio cholerae that is based on a DNA hybridization strategy utilizing nanostructured magnesium oxide (nMgO; size >30 nm) particles. The morphology and size of the synthesized nMgO were determined by transmission electron microscopic (TEM) studies. The probe DNA (pDNA) was conjugated with nMgO and characterized by X-ray photoelectron and Fourier transform infrared spectroscopic techniques. The target complementary genomic DNA (cDNA) isolated from clinical samples of V. cholerae was subjected to DNA hybridization studies using the pDNA-nMgO complex and detection of the cDNA was accomplished by measuring changes in PL intensity. The PL peak intensity measured at 700 nm (red emission) increases with the increase in cDNA concentration. A linear range of response in the developed PL genosensor was observed from 100 to 500 ng/μL with a sensitivity of 1.306 emi/ng, detection limit of 3.133 ng/μL and a regression coefficient (R2) of 0.987. These results show that this ultrasensitive PL genosensor has the potential for applications in the clinical diagnosis of cholera.

Item Type:	Article
Uncontrolled Keywords:	Cholera Detection
Subjects:	Health Care Sciences & Services
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	12 Sep 2016 05:42
Last Modified:	12 Sep 2016 05:42
URI:	http://npl.csircentral.net/id/eprint/1695

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