Singh, Renu and Sumana, G. and Verma, Rachna and Sood, Seema and Sood, K. N. and Gupta, Rajinder K. and Malhotra, B. D. (2010) Fabrication of Neisseria gonorrhoeae biosensor based on chitosan–MWCNT platform. Thin Solid Films, 519 (3). pp. 1135-1140. ISSN 0040-6090

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Abstract

We report results of studies relating to preparation and characterization of DNA electrodes based on the chitosan–MWCNT/ITO nanobiocomposite platform for electrochemical detection of gonorrhoea, the most common bacterial sexually transmitted disease. (STD). This biosensing electrode has been characterized using FT-IR, SEM, cyclic voltammetry and differential pulse voltammetry, etc. The sensing characteristics have been investigated in phosphate buffer saline using methylene blue as the hybridization indicator. This DNA biosensor has a response time of 60 s and is found to be stable for about 4 months when stored at 4 °C. The results of DPV studies reveal that this bioelectrode can detect complementary DNA concentration in a wide range of 1 × 10− 6 M to 1 × 10− 17 M with a detection limit of 1 × 10− 16 M.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Chitosan; Carbon nanotubes; Nanocomposite; Sexually transmitted disease; Neisseria gonorrhoeae; DNA biosensor
Subjects:	Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	19 Apr 2012 06:31
Last Modified:	08 May 2012 12:12
URI:	http://npl.csircentral.net/id/eprint/158

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