Tyagi, Mukta and Chandran, Achu and Joshi, Tilak and Prakash, Jai and Agrawal, V. V. and Biradar, A. M. (2014) Self assembled monolayer based liquid crystal biosensor for free cholesterol detection. Applied Physics Letters, 104 (15). ISSN 0003-6951

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Abstract

A unique cholesterol oxidase (ChOx) liquid crystal (LC) biosensor, based on the disruption of orientation in LCs, is developed for cholesterol detection. A self-assembled monolayer (SAM) of Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP) and (3-Aminopropyl)trimethoxy-silane (APTMS) is prepared on a glass plate by adsorption. The enzyme (ChOx) is immobilized on SAM surface for 12 h before utilizing the film for biosensing purpose. LC based biosensing study is conducted on SAM/ChOx/LC (5CB) cells for cholesterol concentrations ranging from 10 mg/dl to 250 mg/dl. The sensing mechanism has been verified through polarizing optical microscopy, scanning electron microscopy, and spectrometric techniques.

Item Type:	Article
Subjects:	Physics
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	04 Nov 2015 10:46
Last Modified:	04 Nov 2015 10:46
URI:	http://npl.csircentral.net/id/eprint/1522

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