Jangir, Deepak K. and Mehrotra, Ranjana (2014) Raman spectroscopic evaluation of DNA adducts of a platinum containing anticancer drug. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 130. 386-389 . ISSN 1386-1425

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Abstract

Mechanistic understanding of the interaction of drugs with their target molecules is important for better understanding of their mode of action and to improve their efficacy. Carboplatin is a platinum containing anticancer drug, used to treat different type of tumors. In the present work, we applied Raman spectroscopy to study the interaction of carboplatin with DNA at molecular level using different carboplatin-DNA molar ratios. These Raman spectroscopic results provide comprehensive understanding on the carboplatin-DNA interactions and indicate that DNA cross-linked adducts formed by carboplatin are similar to cisplatin adducts. The results indicate that guanine N7 and adenine N7 are the putative sites for carboplatin interaction. It is observed that carboplatin has some affinity toward cytosine in DNA. Phosphate sugar backbone of DNA showed conformation perturbation in DNA which were easily sensible at higher concentrations of carboplatin. Most importantly, carboplatin interaction induces intermediate A- and B-DNA conformations at the cross-linking sites.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Elsevier.
Uncontrolled Keywords:	FT-Raman spectroscopy Carboplatin Calf-thymus DNA Carboplatin–DNA interaction
Subjects:	Genetics & Heredity Pharmacology & Pharmacy Spectroscopy
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	04 Nov 2015 07:04
Last Modified:	04 Nov 2015 07:04
URI:	http://npl.csircentral.net/id/eprint/1491

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