Pandey, Chandra Mouli and Sumana, Gajjala and Tiwari, Ida (2014) Nanostructuring of hierarchical 3D cystine flowers for high-performance electrochemical immunosensor. Biosensors and Bioelectronics, 61. pp. 328-335. ISSN 0956-5663

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Abstract

Here, we report a simple and reproducible method for large scale fabrication of novel flower and palm-leaf like 3D cystine microstructures (CMs) with high uniformity having a size of 50 gm and 10 gm respectively, through a facile aqueous solution route as a function of pH and concentration. In a proof-of-concept study, the 3D CMs have been further explored to fabricate a label-free high-performance electrochemical immunosensor by immobilizing monoclonal antibodies. Electrochemical methods were employed to study the stepwise modification of the system and the electronic transduction for the detection. The fabricated immunosensor design demonstrates high performance with enhanced sensitivity (4.70 cfu ml(-1)) and linear sensing range from 10 to 3 x 10(9) cfu ml(-1) a long shelf-life (35 days) and high selectivity over other bacterial pathogens. The enhanced performance originates from a novel nanostructuring in which the CMs provide higher surface coverage for the immobilization of antibodies providing excellent electronic/ionic conductivity which result in the enhanced sensitivity.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Elsevier.
Uncontrolled Keywords:	Nanostructuring Cystine Organic flowers Immunosensor Electrochemical
Subjects:	Biotechnology & Applied Microbiology Chemistry Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	28 Oct 2015 06:38
Last Modified:	28 Oct 2015 06:38
URI:	http://npl.csircentral.net/id/eprint/1426

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