Dhand, Chetna and Solanki, Pratima R. and Pandey, Manoj K. and Datta, Monika and Malhotra, B. D. (2010) Electrophoretically deposited polyaniline nanotubes based film for cholesterol detection. Electrophoresis, 31 (22). pp. 3754-3762. ISSN 1522-2683

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Abstract

Polyaniline nanotube (PANI-NT) based films have been fabricated onto indium-tin-oxide (ITO) coated glass plates via electrophoretic technique. These PANI-NT/ITO electrodes have been utilized for covalent immobilization of cholesterol oxidase (ChOx) using glutaraldehyde (Glu) as cross-linker. Structural, morphological and electrochemical characterization of PANI-NT/ITO electrode and ChOx/Glu/PANI-NT/ITO bioelectrode have been done using FT-IR spectroscopy, SEM, electrochemical impedance spectroscopy and cyclic voltammetry techniques. Response studies of the ChOx/Glu/PANI-NT/ITO bioelectrode have been carried out using both linear sweep voltammetry and UV-Visible spectrophotometry. The results of the biosensing studies reveal that this bioelectrode can be used to detect cholesterol in wide detection range of 25–500 mg/dL with high sensitivity of 3.36 mA mg−1  dL and fast response time of 30 s at pH 7.4. This bioelectrode exhibits very low value of Michaelis–Menten constant of 1.18 mM indicating enhanced interactions between cholesterol and ChOx immobilized onto this nanostructured PANI matrix.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Uncontrolled Keywords:	Biosensor; Cholesterol; Conducting polymers; Electrophoretic deposition; Polyaniline nanotubes
Subjects:	Biochemistry & Molecular Biology Chemistry Mathematics Medical Laboratory Technology Microscopy
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	13 Apr 2012 06:17
Last Modified:	13 Apr 2012 06:17
URI:	http://npl.csircentral.net/id/eprint/127

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