Dhand, Chetna and Sumana, G. and Datta, Monika and Malhotra, B. D. (2010) Electrophoretically deposited nano-structured polyaniline film for glucose sensing. Thin Solid Films, 519 (3). pp. 1145-1150. ISSN 0040-6090
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Abstract
Electrophoretically deposited nano-structured polyaniline (NS-PANI) film has been utilized for fabrication of glucose biosensor by covalent immobilization of glucose oxidase (GOx) using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. This GOx/NS-PANI/ITO bioelectrode has been characterized using scanning electron microscopy, FT-IR, UV–Visible spectroscopy and differential pulse voltammetry (DPV) techniques. The response studies carried out on GOx/NS-PANI/ITO bioelectrode using DPV and photometric studies reveal linearity up to 400 mgdL− 1 with sensitivity as 1.05 × 10− 4 mA mg− 1 dL and 3.887 × 10− 5 Abs mg− 1 dL, respectively. The lower value of Michaelis–Menten constant obtained for immobilized GOx (2.1 mM) compared with that of free GOx (5.85 mM) suggests high affinity of enzyme to this matrix.
Item Type: | Article |
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Additional Information: | Copyright for this article belongs to M/s Elsevier B.V. |
Uncontrolled Keywords: | Electrophoretic deposition; Nano-structured polyaniline; Glucose oxidase; Biosensor; Conducting polymers |
Subjects: | Materials Science Physics |
Divisions: | UNSPECIFIED |
Depositing User: | Ms Neetu Chandra |
Date Deposited: | 13 Apr 2012 05:58 |
Last Modified: | 13 Apr 2012 05:58 |
URI: | http://npl.csircentral.net/id/eprint/126 |
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