Dhand, Chetna and Sumana, G. and Datta, Monika and Malhotra, B. D. (2010) Electrophoretically deposited nano-structured polyaniline film for glucose sensing. Thin Solid Films, 519 (3). pp. 1145-1150. ISSN 0040-6090

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Abstract

Electrophoretically deposited nano-structured polyaniline (NS-PANI) film has been utilized for fabrication of glucose biosensor by covalent immobilization of glucose oxidase (GOx) using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. This GOx/NS-PANI/ITO bioelectrode has been characterized using scanning electron microscopy, FT-IR, UV–Visible spectroscopy and differential pulse voltammetry (DPV) techniques. The response studies carried out on GOx/NS-PANI/ITO bioelectrode using DPV and photometric studies reveal linearity up to 400 mgdL− 1 with sensitivity as 1.05 × 10− 4 mA mg− 1 dL and 3.887 × 10− 5 Abs mg− 1 dL, respectively. The lower value of Michaelis–Menten constant obtained for immobilized GOx (2.1 mM) compared with that of free GOx (5.85 mM) suggests high affinity of enzyme to this matrix.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Electrophoretic deposition; Nano-structured polyaniline; Glucose oxidase; Biosensor; Conducting polymers
Subjects:	Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	13 Apr 2012 05:58
Last Modified:	13 Apr 2012 05:58
URI:	http://npl.csircentral.net/id/eprint/126

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