Verma, Rachna and Sood, Seema and Singh, Renu and Sumana, Gajjala and Bala, Manju and Sharma, Vinod K. and Samantaray, Jyotish C. and Pandey, Ravindra M. and Malhotra, Bansi D. (2014) Coupling electrochemical response of a DNA biosensor with PCR for Neisseria gonorrhoeae detection. Diagnostic Microbiology and Infectious Disease, 78 (1). 16-23 . ISSN 0732-8893

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Abstract

Early diagnosis of gonococcal infections is important with regard to a patient's health and stage of infection. In this context, we report the development of an opa-gene-based electrochemical DNA biosensor for detection of Neisseria gonorrhoeae by monitoring redox peak of methylene blue indicator. The fabricated biosensor has been shown to be highly sensitive and specific when evaluated with complementary, non-complementary, and 1-base mismatch DNA sequences and polymerase chain reaction (PCR) amplified products (amplicons) of standard strain of N. gonorrhoeae (ATCC49226). The biosensor has been further evaluated using amplicons of known positive and negative clinical samples, and cut-off for positives has been determined using receiver operating characteristic curve. The sensitivity (SN), specificity (SP), positive predictive value, and negative predictive value of the biosensor have been found to be 96.2%, 88.2%, 92.6%, and 93.8%, respectively. We conclude that the combination of PCR amplification with electrochemical detection shows distinct advantage of high SN and increased SP for gonococcal detection.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Elsevier.
Uncontrolled Keywords:	Neisseria gonorrhoeae Gonorrhoea Diagnosis PCR Electrochemical DNA biosensor Methylene Blue
Subjects:	Medical Laboratory Technology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	17 Sep 2015 11:16
Last Modified:	17 Sep 2015 11:16
URI:	http://npl.csircentral.net/id/eprint/1214

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